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endothelial cell marker  (Bioss)


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    Structured Review

    Bioss endothelial cell marker
    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular <t>endothelial</t> growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
    Endothelial Cell Marker, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endothelial cell marker/product/Bioss
    Average 95 stars, based on 112 article reviews
    endothelial cell marker - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement"

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    Journal: Biology

    doi: 10.3390/biology15020187

    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
    Figure Legend Snippet: Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Techniques Used: Immunofluorescence, Staining, Double Immunofluorescence Staining

    Vascular endothelial growth factor (VEGF) expression of senescent cells during OTM. ( A ) Representative p16 and VEGF double immunofluorescence staining images. p16/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16 + and VEGF + . ( D ) Quantitative analysis of p16 + VEGF + /VEGF + area in PDL. ( E ) Quantitative analysis of p16 + VEGF + /p16 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns; not significant, ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s test for ( B , D ), and Welch’s ANOVA with Games–Howell post hoc test for ( E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.
    Figure Legend Snippet: Vascular endothelial growth factor (VEGF) expression of senescent cells during OTM. ( A ) Representative p16 and VEGF double immunofluorescence staining images. p16/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16 + and VEGF + . ( D ) Quantitative analysis of p16 + VEGF + /VEGF + area in PDL. ( E ) Quantitative analysis of p16 + VEGF + /p16 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns; not significant, ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s test for ( B , D ), and Welch’s ANOVA with Games–Howell post hoc test for ( E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Techniques Used: Expressing, Double Immunofluorescence Staining, Staining

    Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.
    Figure Legend Snippet: Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Techniques Used: Immunofluorescence, Staining

    Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).
    Figure Legend Snippet: Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).

    Techniques Used:

    VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.
    Figure Legend Snippet: VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Techniques Used: Expressing, Immunofluorescence, Staining



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    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative <t>CD31</t> immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
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    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative <t>CD31</t> immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
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    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative <t>CD31</t> immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
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    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative <t>CD31</t> immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.
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    Image Search Results


    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Immunofluorescence, Staining, Double Immunofluorescence Staining

    Vascular endothelial growth factor (VEGF) expression of senescent cells during OTM. ( A ) Representative p16 and VEGF double immunofluorescence staining images. p16/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16 + and VEGF + . ( D ) Quantitative analysis of p16 + VEGF + /VEGF + area in PDL. ( E ) Quantitative analysis of p16 + VEGF + /p16 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns; not significant, ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s test for ( B , D ), and Welch’s ANOVA with Games–Howell post hoc test for ( E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Vascular endothelial growth factor (VEGF) expression of senescent cells during OTM. ( A ) Representative p16 and VEGF double immunofluorescence staining images. p16/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16 + and VEGF + . ( D ) Quantitative analysis of p16 + VEGF + /VEGF + area in PDL. ( E ) Quantitative analysis of p16 + VEGF + /p16 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns; not significant, ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA with Tukey’s test for ( B , D ), and Welch’s ANOVA with Games–Howell post hoc test for ( E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Expressing, Double Immunofluorescence Staining, Staining

    Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Immunofluorescence, Staining

    Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques:

    VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Expressing, Immunofluorescence, Staining

    Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Dynamic changes in angiogenesis induced by OTM. ( A ) Representative CD31 immunofluorescence staining images. CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of CD31 + area in PDL. ( C ) Representative CD31/vascular endothelial growth factor (VEGF) double immunofluorescence staining images. CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of VEGF + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Immunofluorescence, Staining, Double Immunofluorescence Staining

    Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Cellular senescence of vascular endothelial cells (ECs) induced by OTM. ( A ) Representative p21 and CD31 immunofluorescence staining images. p21/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p21 + CD31 + area in PDL. ( C ) Representative p16 and CD31 immunofluorescence staining images. p16/CD31 staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( D ) Quantitative analysis of p16 + CD31 + area in PDL. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; Kruskal–Wallis with Dunn’s post hoc test. PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Immunofluorescence, Staining

    Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: Overlapping analysis of senescent and endothelial markers during OTM. ( A ) Schematic illustration of the overlapping analysis for p21 and CD31 in PDL. ( B ) Quantitative analysis of p21 + CD31 + area/p21 + area. ( C ) Quantitative analysis of p21 + CD31 + area/CD31 + area. ( D ) Schematic illustration of the overlapping analysis for p16 and CD31 in PDL. ( E ) Quantitative analysis of p16 + CD31 + area/p16 + area. ( F ) Quantitative analysis of p16 + CD31 + area/CD31 + area. Data are mean ± SD (n = 4). ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; Welch’s ANOVA with Games–Howell post hoc test for ( B ), one-way ANOVA with Tukey’s test for ( C , F ), and Kruskal–Wallis with Dunn’s post hoc test for ( E ).

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques:

    VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Journal: Biology

    Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement

    doi: 10.3390/biology15020187

    Figure Lengend Snippet: VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.

    Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.

    Techniques: Expressing, Immunofluorescence, Staining